Journal: The American journal of pathology

Article Title: Gene Expression Differences between Ductal Carcinoma in Situ with and without Progression to Invasive Breast Cancer.

doi: 10.1016/j.ajpath.2017.03.012

Figure Lengend Snippet: Figure 2 Staining pattern for each antibody. A and B: CK81 (A) and NOTCH3 (B) are positive in the myoepithelial cells and negative in the neoplastic DCIS cells. C: COL1A1 shows periductal stromal staining. DeG: SCGB1D2 (D) is positive in the cytoplasm of neoplastic DCIS cells, and S100A7 (E) shows nuclear staining. Both CK18 (F) and urokinase plas- minogen activator (G) are positive in the cytoplasm of neoplastic DCIS cells. H: Epidermal growth factor receptor is positive in the myoepithelial cells and negative in the neoplastic DCIS cells. Scale bars Z 50 mm (AeH).

Article Snippet: COL1A1 (catalog number NB600-408; Novus Biologicals, Littleton, CO) showed periductal stromal staining (Figure 2C) in six cases, but no significant difference (P Z 0.317) was observed between both groups. etween Pure DCIS and DCIS with Synchronous IBC SCGB1D2 (lipophilin B; catalog number NBP1-81304; Novus Biologicals) was positive in the cytoplasm of neoplastic DCIS cells (Figure 2D) and was scored dichotomous (negative/weak or moderate/strong).

Techniques: Staining

Journal: Cell Proliferation

Article Title: Inhibition of metastasis‐associated gene 1 expression affects proliferation and osteogenic differentiation of immortalized human mesenchymal stem cells

doi: 10.1111/j.1365-2184.2010.00735.x

Figure Lengend Snippet: Effect of MTA1 gene silencing on osteogenic marker gene expression under non‐osteogenic conditions assessed by quantitative real‐time RT‐PCR. Scrambled (SCR)‐shRNA‐ and MTA1‐shRNA‐transfected MSCs were seeded in six‐well plates and grown under non‐osteogenic growth conditions for the indicated time periods. Total RNA was extracted and measured by real‐time RT‐PCR for (a) bone sialoprotein (BSP), (b) osteopontin (OSP), (c) Runx2, (d) osteocalcin (OC), (e) collagen type I (Col1A) and (f) alkaline phosphatase (ALP) mRNA expression. mRNA expression levels were normalized to GAPDH. Results are expressed as mean arbitrary units ± SD (n = 3). Asterisks represent statistically significant differences between the groups (*P < 0.05; **P < 0.005; ***P < 0.0005).

Article Snippet: : Hs01076765_g1) and bone sialoprotein (BSP; cat.‐no.

Techniques: Marker, Gene Expression, Quantitative RT-PCR, shRNA, Transfection, Expressing

Journal: Cell Proliferation

Article Title: Inhibition of metastasis‐associated gene 1 expression affects proliferation and osteogenic differentiation of immortalized human mesenchymal stem cells

doi: 10.1111/j.1365-2184.2010.00735.x

Figure Lengend Snippet: Effect of MTA1 gene silencing on osteogenic marker gene expression under osteogenic differentiation conditions assessed by quantitative real‐time RT‐PCR. Scrambled (SCR)‐shRNA‐ and MTA1‐shRNA‐transfected MSCs were seeded in six‐well plates and grown under osteogenic growth conditions for indicated time periods. Total RNA was extracted and measured by real‐time RT‐PCR for (a) bone sialoprotein (BSP), (b) osteopontin (OSP), (c) Runx2, (d) osteocalcin (OC), (e) collagen type I (Col1A) and (f) alkaline phosphatase (ALP) mRNA expression. mRNA expression levels were normalized to GAPDH. Results are expressed as mean arbitrary units ± SD (n = 3). Asterisks represent statistically significant differences between the groups (*P < 0.05; **P < 0.005; ***P < 0.0005).

Article Snippet: : Hs01076765_g1) and bone sialoprotein (BSP; cat.‐no.

Techniques: Marker, Gene Expression, Quantitative RT-PCR, shRNA, Transfection, Expressing

Journal: PLOS ONE

Article Title: BushenHuoxue formula promotes osteogenic differentiation via affecting Hedgehog signaling pathway in bone marrow stem cells to improve osteoporosis symptoms

doi: 10.1371/journal.pone.0289912

Figure Lengend Snippet: The concentration of ALP (A) , BMP (B) and COL1A1 (C) in BMSCs after BSHX, osteogenic inducer, or estradiol treatment.

Article Snippet: The levels of SHH, IHH, Gli2, and Runx2 in bone tissue and levels of alkaline phosphatase (ALP), Bone Morphogenetic Protein (BMP), and type I collagen (COL1a1) in the culture supernatant were determined using a quantitative sandwich technique, for which ELISA kits (eBioscience, US) were used according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: JOR Spine

Article Title: In vitro nucleus pulposus tissue model with physicochemical stresses

doi: 10.1002/jsp2.1105

Figure Lengend Snippet: Gene expression profiles and immunohistology of nucleus pulposus (NP) cells/clusters in response to changes in cyclic hydrostatic pressure (HP) followed by constant HP in high (450 mOsm/kg H 2 O) or low (320 mOsm/kg H 2 O) osmolality. A, The expression of aggrecan core protein ( Acan) , collagen type‐I ( Col1a1 ), and collagen type‐II ( Col2a1 ). RQ, relative quantity. Mean ± SD ( n = 3). B, The accumulation of keratan sulfate (KS) in brown and counterstained with hematoxylin. Arrows indicate intense accumulation of KS and circles indicate empty spaces. C, The accumulation of collagen type‐I (Col‐1) in black and counterstained with Contrast Red. D, The accumulation of collagen type‐II (Col‐2) in brown and counterstained with hematoxylin. Arrows indicate intense accumulation of Col‐2 and circles indicate empty spaces. Each section is 7 μm thick and a bar indicates 100 μm

Article Snippet: The TaqMan primers were a Col1a1 : Bt03225358_g1; Col2a1 : Bt03251837_mH; Acan : Bt03212189_m1; Mmp‐13 : Bt03214051_m1; and GAPDH : Bt03210919_g1 (Life Technology, Carlsbad, California).

Techniques: Gene Expression, Expressing

Journal: JOR Spine

Article Title: In vitro nucleus pulposus tissue model with physicochemical stresses

doi: 10.1002/jsp2.1105

Figure Lengend Snippet: Gene expression profiles and immunohistology of nucleus pulposus (NP) cells/clusters in response to two rounds of repetitive changes in cyclic hydrostatic pressure (HP) applied for 2 days followed by constant HP for 1 day in high osmolality (450 mOsm/kg H 2 O). A, RQ of the expression of aggrecan core protein ( Acan ), collagen type‐I ( Col1a1 ), and collagen type‐II ( Col2a1 ) and matrix metalloproteinase‐13 ( Mmp13 ). RQ: relative quantity. Mean ± SD (n = 6). *: P < .05. B, The accumulation of keratan sulfate (KS) in brown and counterstained with hematoxylin. Arrows indicate intense accumulation of KS and circles indicate empty spaces. The accumulation of collagen type‐1 (Col‐1) and metalloproteinase‐13 (MMP‐13) in black and counterstained with Contrast Red. Arrows indicate intense accumulation of KS and MMP‐13. Each section is 7 μm thick and a bar indicates 100 μm

Article Snippet: The TaqMan primers were a Col1a1 : Bt03225358_g1; Col2a1 : Bt03251837_mH; Acan : Bt03212189_m1; Mmp‐13 : Bt03214051_m1; and GAPDH : Bt03210919_g1 (Life Technology, Carlsbad, California).

Techniques: Gene Expression, Expressing